Metabolic profiles from two different breakfast meals characterized by 1H NMR-based metabolomics

Millie Rådjursöga; Göran B Karlsson; Helen M Lindqvist; Anders Pedersen; Cecilia Persson; Rui Climaco Pinto; Lars Ellegård; Anna Winkvist

doi:10.1016/j.foodchem.2017.03.142

Metabolic profiles from two different breakfast meals characterized by ¹H NMR-based metabolomics

Food Chem. 2017 Sep 15:231:267-274. doi: 10.1016/j.foodchem.2017.03.142. Epub 2017 Mar 27.

Authors

Millie Rådjursöga¹, Göran B Karlsson², Helen M Lindqvist³, Anders Pedersen⁴, Cecilia Persson⁵, Rui Climaco Pinto⁶, Lars Ellegård⁷, Anna Winkvist⁸

Affiliations

¹ Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy, University of Gothenburg, Box 459, 405 30 Gothenburg, Sweden. Electronic address: [email protected].
² Swedish NMR Centre, University of Gothenburg, Box 465, 405 30 Gothenburg, Sweden. Electronic address: [email protected].
³ Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy, University of Gothenburg, Box 459, 405 30 Gothenburg, Sweden. Electronic address: [email protected].
⁴ Swedish NMR Centre, University of Gothenburg, Box 465, 405 30 Gothenburg, Sweden. Electronic address: [email protected].
⁵ Swedish NMR Centre, University of Gothenburg, Box 465, 405 30 Gothenburg, Sweden. Electronic address: [email protected].
⁶ Computational Life Science Cluster, Department of Chemistry, Umeå University, 90187 Umeå, Sweden; Bioinformatics Infrastructure for Life Sciences (BILS), Linköping, Sweden. Electronic address: [email protected].
⁷ Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy, University of Gothenburg, Box 459, 405 30 Gothenburg, Sweden. Electronic address: [email protected].
⁸ Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy, University of Gothenburg, Box 459, 405 30 Gothenburg, Sweden. Electronic address: [email protected].

PMID: 28450006
DOI: 10.1016/j.foodchem.2017.03.142

Abstract

It is challenging to measure dietary exposure with techniques that are both accurate and applicable to free-living individuals. We performed a cross-over intervention, with 24 healthy individuals, to capture the acute metabolic response of a cereal breakfast (CB) and an egg and ham breakfast (EHB). Fasting and postprandial urine samples were analyzed using ¹H nuclear magnetic resonance (NMR) spectroscopy and multivariate data analysis. Metabolic profiles were distinguished in relation to ingestion of either CB or EHB. Phosphocreatine/creatine and citrate were identified at higher concentrations after consumption of EHB. Beverage consumption (i.e., tea or coffee) could clearly be seen in the data. 2-furoylglycine and 5-hydroxymethyl-2-furoic acid - potential biomarkers for coffee consumption were identified at higher concentrations in coffee drinkers. Thus ¹H NMR urine metabolomics is applicable in the characterization of acute metabolic fingerprints from meal consumption and in the identification of metabolites that may serve as potential biomarkers.

Keywords: (1)H NMR; Biomarker; Metabolic phenotype; Metabolomics; Nutrition; Urine.

MeSH terms

Biomarkers
Breakfast*
Humans
Magnetic Resonance Spectroscopy
Metabolome
Metabolomics*
Postprandial Period*

Substances

Biomarkers