Phenotypic and Molecular Evaluation of Echinocandin Susceptibility of Candida glabrata, Candida bracarensis, and Candida nivariensis Strains Isolated during 30 Years in Argentina

Antimicrob Agents Chemother. 2017 Jun 27;61(7):e00170-17. doi: 10.1128/AAC.00170-17. Print 2017 Jul.

Abstract

The echinocandin susceptibilities of 122 Candida glabrata complex strains (including 5 Candida nivariensis and 3 Candida bracarensis strains) were evaluated by microdilution and compared with the results from a molecular tool able to detect FKS mutations. No echinocandin resistance was detected. The PCR results coincide with the MIC data in 99.25% of the cases (1 C. glabrata strain was misidentified as resistant) but were 20 h faster. C. nivariensis FKS genes were sequenced and showed differences with C. glabrataFKS genes.

Keywords: Candida bracarensis; Candida glabrata species complex; Candida nivariensis; echinocandin resistance; hot spot.

MeSH terms

  • Antifungal Agents / pharmacology*
  • Candida
  • Candida glabrata / drug effects*
  • Candida glabrata / genetics
  • Candidiasis / genetics
  • Drug Resistance, Fungal / genetics
  • Echinocandins / pharmacology*
  • Microbial Sensitivity Tests
  • Mutation / genetics
  • Polymerase Chain Reaction

Substances

  • Antifungal Agents
  • Echinocandins