Epidemiology and whole genome sequencing of an ongoing point-source Salmonella Agona outbreak associated with sushi consumption in western Sydney, Australia 2015

Epidemiol Infect. 2017 Jul;145(10):2062-2071. doi: 10.1017/S0950268817000693. Epub 2017 May 2.

Abstract

During May 2015, an increase in Salmonella Agona cases was reported from western Sydney, Australia. We examine the public health actions used to investigate and control this increase. A descriptive case-series investigation was conducted. Six outbreak cases were identified; all had consumed cooked tuna sushi rolls purchased within a western Sydney shopping complex. Onset of illness for outbreak cases occurred between 7 April and 24 May 2015. Salmonella was isolated from food samples collected from the implicated premise and a prohibition order issued. No further cases were identified following this action. Whole genome sequence (WGS) analysis was performed on isolates recovered during this investigation, with additional S. Agona isolates from sporadic-clinical cases and routine food sampling in New South Wales, January to July 2015. Clinical isolates of outbreak cases were indistinguishable from food isolates collected from the implicated sushi outlet. Five additional clinical isolates not originally considered to be linked to the outbreak were genomically similar to outbreak isolates, indicating the point-source contamination may have started before routine surveillance identified an increase. This investigation demonstrated the value of genomics-guided public health action, where near real-time WGS enhanced the resolution of the epidemiological investigation.

Keywords: Salmonella Agona; Outbreak; public health action; sushi; whole genome sequencing.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Child
  • Child, Preschool
  • Disease Outbreaks*
  • Fish Products / microbiology*
  • Genome, Bacterial*
  • Humans
  • Middle Aged
  • New South Wales / epidemiology
  • Salmonella Food Poisoning / epidemiology*
  • Salmonella Food Poisoning / microbiology
  • Salmonella enterica / genetics
  • Salmonella enterica / physiology*
  • Sequence Analysis, DNA
  • Young Adult