The structure-function relationships of insulin-like growth factor 1 Ec in C2C12 cells

Qian Yi; Jianguo Feng; Ling He; Rongxue Wan; Hui Zeng; Li Yang; Shiyong Wu; Liling Tang

doi:10.1080/19336918.2017.1318240

The structure-function relationships of insulin-like growth factor 1 Ec in C2C12 cells

Cell Adh Migr. 2018 Jan 2;12(1):47-55. doi: 10.1080/19336918.2017.1318240. Epub 2017 May 12.

Authors

Qian Yi^{1

2}, Jianguo Feng^{1

3}, Ling He¹, Rongxue Wan¹, Hui Zeng¹, Li Yang¹, Shiyong Wu⁴, Liling Tang¹

Affiliations

¹ a Key Laboratory of Biorheological Science and Technology , Ministry of Education, College of Bioengineering, Chongqing University , Chongqing , China.
² d Department of Biomedical Engineering , The State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and MOST, Chongqing Medical University , Chongqing , China.
³ c Department of Anesthesiology , The Affiliated Hospital of Southwest Medical University , Luzhou , Sichuan Province , China.
⁴ b Edison Biotechnology Institute and Department of Chemistry and Biochemistry, Ohio University , Athens , OH , USA.

Abstract

Insulin-like growth factor 1 (IGF1) is a crucial growth factor, that regulates skeletal muscles development during cell growth and repair. Recently, its alternative splicing variant, named IGF1Ec, also named mechano-growth factor (MGF), has gained attentions as a new damage repair factor. However, the structure-function relationships of IGF1Ec have not been fully clarified due to contradictory reports. In this study, we systematically investigated physiologic responses of C2C12 muscle cells to IGF1Ec, IGF1 and MGF E peptide. Our data indicate that while the N-terminal sequence of IGF1Ec, which is homolog in part with IGF1, promotes proliferation; the C-terminal sequence of IGF1Ec, which is identical to MGF E, promotes differentiation and migration of C2C12 cells. Our results suggest that MGF E cannot completely replace all the functions of IGF1Ec on muscle repair and regeneration, and elucidate the relationships between structure and function of IGF1Ec.

Keywords: C2C12 cells; IGF1; IGF1Ec; IGF1–24; MGF E; differentiation; migration; proliferation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation / physiology*
Cell Line
Cell Movement / physiology
Cell Proliferation / physiology*
Insulin-Like Growth Factor I / metabolism*
Mice
Muscle, Skeletal / metabolism*
Structure-Activity Relationship

Substances

insulin-like growth factor-1, mouse
Insulin-Like Growth Factor I

Grants and funding

This study is supported by Natural Science Foundation of China under Grant 31670952 and 30970701; Fundamental Research Funds for the Central Universities under Grant CDJXS102300 and “111” Project under Grant B06023, China.