We report a case of redissection of the aortic root after graft replacement for acute aortic dissection using BioGlue to approximate the false lumen. A 49-year-old man underwent graft replacement of the ascending aorta for acute aortic dissection. In this operation, BioGlue was applied to the false lumen of the aortic root. Three months later, computed tomography revealed an ulcer-like projection and an intramural hematoma in the aortic root. Aortic root replacement was performed, in which operative findings showed a new intimal tear and a patent false lumen contained with hematomas and a fragment of BioGlue. Histological examination showed no evidence of adhesion between the intimal media and the adventitia of the aorta, or toxic effect of BioGlue. We conclude that care should be taken in applying the BioGlue to a false lumen and that cautious follow-up is mandatory not to overlook redissection.