Characterization of porcine Trueperella pyogenes by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), molecular typing and antimicrobial susceptibility profiling in Sao Paulo State

Luisa Z Moreno; Carlos E C Matajira; Barbara L P da Costa; Thais S P Ferreira; Givago F R Silva; Mauricio C Dutra; Vasco T M Gomes; Ana Paula S Silva; Ana Paula G Christ; Maria Inês Z Sato; Andrea M Moreno

doi:10.1016/j.cimid.2017.03.005

Characterization of porcine Trueperella pyogenes by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), molecular typing and antimicrobial susceptibility profiling in Sao Paulo State

Comp Immunol Microbiol Infect Dis. 2017 Apr:51:49-53. doi: 10.1016/j.cimid.2017.03.005. Epub 2017 Mar 29.

Affiliations

¹ Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Av. Prof. Dr. Orlando Marques de Paiva, 87, Butantã, 05508-270 São Paulo, SP, Brazil.
² Companhia Ambiental do Estado de São Paulo (CETESB), Av. Prof. Frederico Hermann Jr, 345, Pinheiros, 05489 900 São Paulo, SP, Brazil.
³ Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Av. Prof. Dr. Orlando Marques de Paiva, 87, Butantã, 05508-270 São Paulo, SP, Brazil. Electronic address: [email protected].

PMID: 28504095
DOI: 10.1016/j.cimid.2017.03.005

Abstract

Trueperella pyogenes can be found as a commensal or pathogenic bacterium among animals causing a variety of pyogenic infections in several species. The agent appears to act primarily as an opportunistic pathogen but may disseminate and produce metastatic abscesses accompanied or not by mastitis, metritis or pneumonia. In this study, 30 porcine T. pyogenes strains were identified by MALDI-TOF MS and 16S rRNA sequencing and further evaluated in relation to their resistance and genetic profiles through broth microdilution and single enzyme AFLP. All strains were susceptible to β-lactams, florfenicol, gentamicin, spectinomycin and tiamulin. The highest resistance rates were observed for sulfonamides, tetracyclines and clindamycin. All isolates could be characterized by SE-AFLP presenting more than 80% of similarity, despite their distinct origins. Four genotypes were detected with the segregation of T. pyogenes ATCC 19411 from Brazilian T. pyogenes strains. No correlation between genotypes and isolates origins and susceptibility profile was observed.

Keywords: Antimicrobial resistance; MALDI-TOF; Sequencing; Swine; T. pyogenes.

MeSH terms

Actinomycetaceae / drug effects*
Actinomycetaceae / genetics*
Actinomycetaceae / isolation & purification
Actinomycetales Infections / epidemiology
Actinomycetales Infections / microbiology*
Amplified Fragment Length Polymorphism Analysis
Animals
Anti-Bacterial Agents / pharmacology*
Bacterial Typing Techniques*
Brazil / epidemiology
Drug Resistance, Multiple, Bacterial
Genotype
Microbial Sensitivity Tests
Molecular Typing / methods*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Swine

Substances

Anti-Bacterial Agents
RNA, Ribosomal, 16S