CRISPR/Cas system for yeast genome engineering: advances and applications

Vratislav Stovicek; Carina Holkenbrink; Irina Borodina

doi:10.1093/femsyr/fox030

CRISPR/Cas system for yeast genome engineering: advances and applications

FEMS Yeast Res. 2017 Aug 1;17(5):fox030. doi: 10.1093/femsyr/fox030.

Authors

Vratislav Stovicek¹, Carina Holkenbrink¹, Irina Borodina¹

Affiliation

¹ The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.

Abstract

The methods based on the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system have quickly gained popularity for genome editing and transcriptional regulation in many organisms, including yeast. This review aims to provide a comprehensive overview of CRISPR application for different yeast species: from basic principles and genetic design to applications.

Keywords: CRISPR interference; CRISPR transcriptional regulation; CRISPR/Cas; Saccharomyces cerevisiae; genome editing; yeasts.

Publication types

Review

MeSH terms

CRISPR-Cas Systems*
Gene Editing / methods*
Genetics, Microbial / methods*
Metabolic Engineering / methods*
Saccharomyces cerevisiae / genetics*