Clonal Dissemination of OXA-232 Carbapenemase-Producing Klebsiella pneumoniae in Neonates

Dandan Yin; Dong Dong; Ke Li; Lei Zhang; Jianliang Liang; Yang Yang; Nana Wu; Yiyan Bao; Chuanqing Wang; Fupin Hu

doi:10.1128/AAC.00385-17

Clonal Dissemination of OXA-232 Carbapenemase-Producing Klebsiella pneumoniae in Neonates

Antimicrob Agents Chemother. 2017 Jul 25;61(8):e00385-17. doi: 10.1128/AAC.00385-17. Print 2017 Aug.

Authors

Dandan Yin¹, Dong Dong^{2

3}, Ke Li⁴, Lei Zhang¹, Jianliang Liang¹, Yang Yang², Nana Wu¹, Yiyan Bao¹, Chuanqing Wang⁵, Fupin Hu^{6

3}

Affiliations

¹ Clinical Microbiology Laboratory, Department of Nosocomial Infection Control, Children's Hospital of Fudan University, Shanghai, China.
² Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.
³ Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, China.
⁴ Department of Laboratory Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
⁵ Clinical Microbiology Laboratory, Department of Nosocomial Infection Control, Children's Hospital of Fudan University, Shanghai, China [email protected] [email protected].
⁶ Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China [email protected] [email protected].

Abstract

Five OXA-232 carbapenemase-producing Klebsiella pneumoniae isolates, belonging to the pandemic clone sequence type 15 (ST15), were isolated from neonates and coproduced bla_CTX-M-15 and bla_SHV-1 genes. All isolates were resistant to ertapenem (MICs of >32 μg/ml) and meropenem (MICs of 4 to 8 μg/ml) and susceptible or intermediate to imipenem (MICs of 1 to 2 μg/ml). The bla_OXA-232 gene was located on a ColE-type transformable plasmid of 6,141 bp. To the best of our knowledge, this is the first report of OXA-232 carbapenemase among clinical isolates in China.

Keywords: Klebsiella pneumoniae; OXA-232; ST15; clonal dissemination; neonates.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
China
Drug Resistance, Multiple, Bacterial / genetics*
Ertapenem
Humans
Imipenem / pharmacology
Klebsiella Infections / drug therapy
Klebsiella Infections / microbiology
Klebsiella pneumoniae / drug effects*
Klebsiella pneumoniae / enzymology
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / isolation & purification
Meropenem
Microbial Sensitivity Tests
Plasmids / genetics
Thienamycins / pharmacology
beta-Lactamases / genetics*
beta-Lactamases / metabolism
beta-Lactams / pharmacology

Substances

Anti-Bacterial Agents
Bacterial Proteins
Thienamycins
beta-Lactams
Imipenem
beta-lactamase CTX-M-15
beta-lactamase PIT-2
beta-Lactamases
beta-lactamase OXA-232, Klebsiella pneumoniae
carbapenemase
Meropenem
Ertapenem