Synergistic bactericidal activity of chlorhexidine-loaded, silver-decorated mesoporous silica nanoparticles

Int J Nanomedicine. 2017 May 9:12:3577-3589. doi: 10.2147/IJN.S133846. eCollection 2017.

Abstract

Combination of chlorhexidine (CHX) and silver ions could engender synergistic bactericidal effect and improve the bactericidal efficacy. It is highly desired to develop an efficient carrier for the antiseptics codelivery targeting infection foci with acidic microenvironment. In this work, monodisperse mesoporous silica nanoparticle (MSN) nanospheres were successfully developed as an ideal carrier for CHX and nanosilver codelivery through a facile and environmentally friendly method. The CHX-loaded, silver-decorated mesoporous silica nanoparticles (Ag-MSNs@CHX) exhibited a pH-responsive release manner of CHX and silver ions simultaneously, leading to synergistically antibacterial effect against both gram-positive Staphylococcus aureus and gram-negative Escherichia coli. Moreover, the effective antibacterial concentration of Ag-MSNs@CHX showed less cytotoxicity on normal cells. Given their synergistically bactericidal ability and good biocompatibility, these nanoantiseptics might have effective and broad clinical applications for bacterial infections.

Keywords: chlorhexidine; good biocompatibility; mesoporous silica nanoparticles; pH-responsive release; silver nanoparticles; synergistic bactericidal activity.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / pharmacology*
  • Chlorhexidine / administration & dosage
  • Chlorhexidine / chemistry
  • Chlorhexidine / pharmacology*
  • Drug Delivery Systems / methods
  • Drug Synergism
  • Escherichia coli / drug effects
  • Human Umbilical Vein Endothelial Cells / drug effects
  • Humans
  • Hydrogen-Ion Concentration
  • Mice
  • Microbial Sensitivity Tests
  • NIH 3T3 Cells / drug effects
  • Nanoparticles / administration & dosage
  • Nanoparticles / chemistry*
  • Nanospheres / administration & dosage
  • Nanospheres / chemistry
  • Silicon Dioxide / chemistry
  • Silver / chemistry
  • Silver / pharmacology*
  • Staphylococcus aureus / drug effects

Substances

  • Anti-Bacterial Agents
  • Silver
  • Silicon Dioxide
  • Chlorhexidine