Cis-perturbation of cancer drivers by the HTLV-1/BLV proviruses is an early determinant of leukemogenesis

Nat Commun. 2017 May 23:8:15264. doi: 10.1038/ncomms15264.

Abstract

Human T-cell leukaemia virus type-1 (HTLV-1) and bovine leukaemia virus (BLV) infect T- and B-lymphocytes, respectively, provoking a polyclonal expansion that will evolve into an aggressive monoclonal leukaemia in ∼5% of individuals following a protracted latency period. It is generally assumed that early oncogenic changes are largely dependent on virus-encoded products, especially TAX and HBZ, while progression to acute leukaemia/lymphoma involves somatic mutations, yet that both are independent of proviral integration site that has been found to be very variable between tumours. Here, we show that HTLV-1/BLV proviruses are integrated near cancer drivers which they affect either by provirus-dependent transcription termination or as a result of viral antisense RNA-dependent cis-perturbation. The same pattern is observed at polyclonal non-malignant stages, indicating that provirus-dependent host gene perturbation contributes to the initial selection of the multiple clones characterizing the asymptomatic stage, requiring additional alterations in the clone that will evolve into full-blown leukaemia/lymphoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Carcinogenesis / genetics*
  • Cattle
  • Female
  • Genome
  • Host-Pathogen Interactions / genetics
  • Human T-lymphotropic virus 1 / genetics
  • Human T-lymphotropic virus 1 / physiology*
  • Humans
  • Leukemia / genetics*
  • Leukemia / virology*
  • Leukemia Virus, Bovine / genetics
  • Leukemia Virus, Bovine / physiology*
  • Male
  • Models, Biological
  • Proviruses / genetics
  • Proviruses / physiology*
  • RNA, Antisense / metabolism
  • Sheep
  • Transcription, Genetic
  • Virus Integration / genetics

Substances

  • RNA, Antisense