Gardnerella vaginalis bacteremia associated with severe acute encephalopathy in a young female patient

Anaerobe. 2017 Oct:47:132-134. doi: 10.1016/j.anaerobe.2017.05.010. Epub 2017 May 22.

Abstract

Gardnerella vaginalis is a facultative anaerobic bacterium that inhabits the genitourinary tract of both healthy women and those with bacterial vaginosis. We report a case of G. vaginalis bacteremia associated with severe toxic encephalopathy in a young woman. Anaerobic blood cultures yielded pure growth of small gram-variable rods later identified as G. vaginalis by both rapid biochemical tests and 16S rRNA gene sequencing. The patient recovered after treatment with amoxicillin-clavulanate according to the in vitro susceptibility testing. The complete genome of G. vaginalis isolate from blood cultures was determined. In vitro G. vaginalis isolate produced elevated amounts of a pore-forming toxin vaginolysin compared to control G. vaginalis isolates. We hypothesize that this toxin, if produced in high amounts in blood, is able to disrupt the blood-brain barrier and exert a toxic activity on brain cells.

Keywords: Bacteremia; Complete genome; Encephalopathy; Gardnerella vaginalis; Vaginolysin.

Publication types

  • Case Reports

MeSH terms

  • Amoxicillin-Potassium Clavulanate Combination / administration & dosage
  • Anti-Bacterial Agents / administration & dosage
  • Bacteremia / complications*
  • Bacteremia / diagnosis*
  • Bacteremia / drug therapy
  • Bacterial Proteins / analysis
  • Bacterial Toxins / analysis
  • Bacterial Typing Techniques
  • Brain Diseases / etiology*
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Female
  • Gardnerella vaginalis / classification
  • Gardnerella vaginalis / genetics
  • Gardnerella vaginalis / isolation & purification*
  • Gram-Positive Bacterial Infections / complications*
  • Gram-Positive Bacterial Infections / diagnosis*
  • Gram-Positive Bacterial Infections / drug therapy
  • Humans
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Treatment Outcome
  • Young Adult

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Bacterial Toxins
  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S
  • vaginolysin, Gardnerella vaginalis
  • Amoxicillin-Potassium Clavulanate Combination