Abstract
A human polymorphonuclear leukocyte (PMN) activating factor (AFH-S) was isolated from a tropical plant seed, Aleurites Fordii Hemsl. (AFH) in PBS. It is found that the AFH-S stimulate human PMNs and induced superoxide generation and chemotaxis. Superoxide generation was affected by the extracellular calcium ion or pretreatment with H-7 (PK-C inhibitor), but not by mepacrine (PLA2 inhibitor) or pertussis toxin (islet-activating protein: IAP). Furthermore, a lag time exists dose-dependently. In addition the cytosolic calcium was not increased by the stimulation with AFH-S. Thus, the receptor for AFH-S was suggested to be independent from Ni-like protein, PI-response, or PLA2-activation, and stimulate PMNs through activation of PK-C.
MeSH terms
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Calcium / metabolism
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Chemotactic Factors / isolation & purification*
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Chemotactic Factors / pharmacology
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Chemotaxis, Leukocyte / drug effects
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Humans
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In Vitro Techniques
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Interleukin-8
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Isoquinolines / pharmacology
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N-Formylmethionine Leucyl-Phenylalanine / pharmacology
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Neutrophils / drug effects
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Neutrophils / immunology*
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Neutrophils / metabolism
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Peptides / isolation & purification*
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Peptides / pharmacology
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Pertussis Toxin
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Piperazines / pharmacology
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Quinacrine / pharmacology
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Seeds / immunology
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Superoxides / metabolism
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Virulence Factors, Bordetella / pharmacology
Substances
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Chemotactic Factors
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Interleukin-8
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Isoquinolines
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Peptides
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Piperazines
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Virulence Factors, Bordetella
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Superoxides
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N-Formylmethionine Leucyl-Phenylalanine
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Pertussis Toxin
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Quinacrine
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Calcium