Immunogenicity in mice of the sequential polypeptides (Ala-Tyr-Glu-Gly)n, (Ala-Glu-Tyr-Gly)n, (Glu-Ala-Tyr-Gly)n and (Glu-Tyr-Ala-Gly)n and their linkage with the major histocompatibility complex

Exp Clin Immunogenet. 1986;3(4):201-7.

Abstract

The immunogenicity of the four sequential polymers of alpha-L-amino acids referred to below was studied in inbred strains of mice. The responses were linked to the H-2 haplotype, and the Ir genes controlling the responses were mapped to the IA region. The responses were restricted to two haplotypes as follows: (Ala-Tyr-Glu-Gly)n and (Ala-Glu-Tyr-Gly)n - H-2k; (Glu-Ala-Tyr-Gly)n - H-2b; (Glu-Tyr-Ala-Gly)n - H-2k and b. The absence of reciprocal crossreactions among all of these polymers, plus the sequential polymers (Tyr-Ala-Glu-Gly)n and (Tyr-Glu-Ala-Gly)n at both the antibody and T-cell levels, indicated that each of these six polymers was indeed structurally unique.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibody Formation
  • Genetic Linkage
  • Haplotypes
  • Histocompatibility Antigens Class II / genetics*
  • Histocompatibility Antigens Class II / immunology
  • Major Histocompatibility Complex*
  • Mice
  • Mice, Inbred Strains / genetics
  • Mice, Inbred Strains / immunology*
  • Molecular Sequence Data
  • Peptides / immunology*
  • Receptors, Antigen, T-Cell / genetics*
  • Receptors, Antigen, T-Cell / isolation & purification
  • T-Lymphocytes / immunology

Substances

  • Histocompatibility Antigens Class II
  • Peptides
  • Receptors, Antigen, T-Cell
  • poly(glutamyl-tyrosyl-alanyl-glycine)
  • poly(alanyl-glutamyl-tyrosyl-glycine)
  • poly(alanyl-tyrosyl-glutamyl-glycine)
  • poly(glutamyl-alanyl-tyrosyl-glycine)