Reduction and disappearance of anomalous fluorescence patterns by means of absorption of HIV and EBV positive sera to guinea pig kidney and calf erythrocytes: a preliminary study

Boll Ist Sieroter Milan. 1988;67(5-6):351-6.

Abstract

Anomalous pictures observed in HIV infected H9 line cells by immunofluorescence technique, were investigated on sera of drug addicts and on controls. Strong fluorescence artifacts were markedly reduced after an overnight incubation at 4 degrees C. A complete disappearance was obtained after absorption to guinea pig kidney and calf erythrocytes of anti EBV positive sera. This result was fully independent of any anti HIV positivity.

Publication types

  • Comparative Study

MeSH terms

  • AIDS Serodiagnosis*
  • Adsorption
  • Animals
  • Antibodies, Viral / blood*
  • Blotting, Western
  • Cattle
  • Cell Line
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Erythrocytes / immunology*
  • False Positive Reactions
  • Fluorescent Antibody Technique*
  • Guinea Pigs
  • HIV Antibodies / blood*
  • HIV Infections / complications
  • HIV Infections / diagnosis*
  • Hepatitis B / complications
  • Hepatitis B / diagnosis
  • Hepatitis B Antibodies / blood
  • Herpesviridae Infections / complications
  • Herpesviridae Infections / diagnosis
  • Herpesvirus 4, Human / immunology*
  • Humans
  • Kidney / cytology
  • Kidney / immunology*
  • Substance Abuse, Intravenous / blood
  • Substance Abuse, Intravenous / complications

Substances

  • Antibodies, Viral
  • HIV Antibodies
  • Hepatitis B Antibodies