Abstract
A membrane receptor and a cytosolic receptor for somatostatin were found in a human undifferentiated pancreatic cancer cell line (MIA PaCa-2). Binding of somatostatin to this membrane receptor activates dephosphorylation of a phosphotyrosyl-membrane protein whose phosphorylation was promoted by epidermal growth factor (EGF). Vanadate, a purported inhibitor of dephosphorylation, interferes with the action of somatostatin. These findings suggest a possible biochemical mechanism by which somatostatin may inhibit the growth of human pancreatic cancers.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Epidermal Growth Factor / pharmacology
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Humans
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Membrane Proteins / metabolism*
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Pancreatic Neoplasms / metabolism*
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Phosphoprotein Phosphatases / metabolism
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Phosphoproteins / metabolism*
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Phosphorylation
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Receptors, Cell Surface / drug effects
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Receptors, Cell Surface / physiology
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Receptors, Somatostatin
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Somatostatin / analogs & derivatives
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Somatostatin / metabolism
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Somatostatin / pharmacology*
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Vanadates
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Vanadium / pharmacology
Substances
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Membrane Proteins
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Phosphoproteins
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Receptors, Cell Surface
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Receptors, Somatostatin
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Vanadium
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Vanadates
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Somatostatin
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somatostatin, N-Tyr(1)-
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Epidermal Growth Factor
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Phosphoprotein Phosphatases