Long non-coding RNA PCAT-1 over-expression promotes proliferation and metastasis in gastric cancer cells through regulating CDKN1A

Gene. 2017 Aug 30:626:337-343. doi: 10.1016/j.gene.2017.05.049. Epub 2017 May 30.

Abstract

Prostate cancer-associated transcript1 (PCAT-1) is a novel lncRNA that involved in cell apoptosis and proliferation of prostate cancer. However, the role of PCAT-1 on gastric cancer remains unclear. In the present study, we found that PCAT-1 was increased in gastric cancer tissues and cell lines. Over-expression of PCAT-1 was correlated with poor overall survival in gastric cancer patients. PCAT-1 knockdown through shRNA in AGS and MGC-803 cells inhibited cell proliferation, migration and invasion by regulating CDKN1A. Our data suggested that PCAT-1 could play an oncogenic role in gastric cancer progression. Silencing PCAT-1 is a potential novel therapeutic approach for gastric cancer.

Keywords: CDKN1A; Gastric cancer; PCAT-1; lncRNA.

MeSH terms

  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation*
  • Cyclin-Dependent Kinase Inhibitor p21 / genetics*
  • Cyclin-Dependent Kinase Inhibitor p21 / metabolism
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Male
  • Middle Aged
  • Neoplasm Metastasis
  • RNA, Long Noncoding / genetics*
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / metabolism
  • Stomach Neoplasms / pathology

Substances

  • CDKN1A protein, human
  • Cyclin-Dependent Kinase Inhibitor p21
  • PCAT-1 lncRNA, human
  • RNA, Long Noncoding