Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells

Judith Olejnik; Emily V Nelson

doi:10.1007/978-1-4939-7116-9_18

Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells

Methods Mol Biol. 2017:1628:227-241. doi: 10.1007/978-1-4939-7116-9_18.

Authors

Judith Olejnik¹, Emily V Nelson²

Affiliations

¹ Department of Microbiology and National Emerging Infectious Diseases Laboratories, School of Medicine, Boston University, Boston, MA, USA. [email protected].
² Department of Microbiology and National Emerging Infectious Diseases Laboratories, School of Medicine, Boston University, Boston, MA, USA.

PMID: 28573624
DOI: 10.1007/978-1-4939-7116-9_18

Abstract

In this chapter, we describe a Western blot assay for the successful detection of apoptosis in Ebola virus (EBOV)-infected cells. The protocol includes all steps from cell culture, infection of cells, generation of lysates, and analysis using Western blot to detect caspase cleavage as marker of apoptosis.

Keywords: Antibodies; Apoptosis; Caspases; Ebola virus; Filoviruses; Western blot; Zaire ebolavirus.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Apoptosis / genetics*
Blotting, Western / methods*
Caspases / genetics
Cell Count
Ebolavirus / genetics*
Ebolavirus / pathogenicity
Hemorrhagic Fever, Ebola / genetics*
Hemorrhagic Fever, Ebola / virology
Humans

Substances

Caspases

Abstract

Publication types

MeSH terms

Substances

Grants and funding