A comparison of enriched culture and Xpert polymerase chain reaction assay of Group B streptococcus carrier status at 35-37-week gestation to labor onset: a prospective controlled study

J Matern Fetal Neonatal Med. 2018 Aug;31(16):2170-2174. doi: 10.1080/14767058.2017.1338255. Epub 2017 Aug 6.

Abstract

Objectives: (1) To compare the accuracy of vagino-rectal enriched culture (EC) and a rapid polymerase chain reaction (PCR) test for the detection of Group B streptococcus (GBS) carrier status at 35-37-week gestation and at onset of labor. (2) To assess the conversion rate of GBS carrier status between 35-37 weeks to the onset of labor according to the EC/PCR tests. A prospective study was performed at a women's health clinic, referred to give birth at one medical center.

Study population: Low risk pregnant women at 35-37-week gestation who did not know their GBS carrier status.

Methods: Participants were evaluated for GBS status both at 35-37 weeks and at labor onset. Correlation between tests was calculated by Spearman correlation.

Results: One hundred and ten specimens were analyzed. Correlations: EC-PCR: 35-37 weeks - very high (r = 0.8), at labor - high (r = 0.5).

Ec-ec: 35-37 weeks and at labor - high (r = 0.39); PCR-PCR: 35-37 weeks and at labor- high (r = 0.7).

Conclusions: Both the EC and Xpert PCR tests are accurate for detecting GBS carrier, both at 35-37 weeks and at labor onset. We did not detect a significant conversion of the GBS status from negative at 35-37 weeks to positive at onset of labor.

Keywords: GBS; PCR; carrier; culture; enriched; prenatal.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adult
  • Bacteriological Techniques / methods*
  • Carrier State
  • Case-Control Studies
  • Female
  • Gestational Age
  • Humans
  • Infectious Disease Transmission, Vertical / prevention & control
  • Labor Onset / physiology
  • Molecular Diagnostic Techniques / methods
  • Polymerase Chain Reaction / methods*
  • Pregnancy
  • Pregnancy Complications, Infectious / diagnosis*
  • Pregnancy Complications, Infectious / microbiology
  • Pregnancy Trimester, Third* / blood
  • Prenatal Diagnosis / methods
  • Rectum / microbiology
  • Sensitivity and Specificity
  • Streptococcal Infections / diagnosis*
  • Streptococcal Infections / microbiology
  • Streptococcal Infections / transmission
  • Streptococcus agalactiae / genetics
  • Streptococcus agalactiae / growth & development*
  • Streptococcus agalactiae / isolation & purification*
  • Vagina / microbiology
  • Young Adult