Rapid Antibiotic Susceptibility Testing for Urinary Tract Infections

Anja Mezger; Mats Nilsson; Dan I Andersson

doi:10.1007/978-1-4939-7037-7_9

Rapid Antibiotic Susceptibility Testing for Urinary Tract Infections

Methods Mol Biol. 2017:1616:147-153. doi: 10.1007/978-1-4939-7037-7_9.

Authors

Anja Mezger^{1

2}, Mats Nilsson³, Dan I Andersson⁴

Affiliations

¹ Broad Institute of MIT and Harvard, Cambridge, MA, USA.
² Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
³ Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden.
⁴ Department of Medical Biochemistry and Microbiology, Uppsala University, Box 256, Uppsala, 751 05, Sweden. [email protected].

PMID: 28600767
DOI: 10.1007/978-1-4939-7037-7_9

Abstract

Antibiotic susceptibility testing is important to guide clinicians in their choice of antibiotic used for treatment of bacterial infections. Current methods are time-consuming and more rapid alternatives are needed. Here, we describe a novel rapid method for antibiotic susceptibility testing which combines phenotypic and genotypic measurements. The use of padlock probes and rolling circle amplification allows for fast and precise determination of antibiotic susceptibilities as well as species identification.

Keywords: Antibiotic susceptibility testing; Padlock probes; Rolling circle amplification; Urinary tract infections.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacteria / drug effects
Bacteria / genetics
DNA Probes
Humans
Microbial Sensitivity Tests / methods*
Nucleic Acid Amplification Techniques / methods
Urinary Tract Infections / microbiology*

Substances

Anti-Bacterial Agents
DNA Probes