A new staining protocol for detection of murine antibody-secreting plasma cell subsets by flow cytometry

Eur J Immunol. 2017 Aug;47(8):1389-1392. doi: 10.1002/eji.201747019. Epub 2017 Jul 13.

Abstract

We provide a robust four-color fluorescence-based flow cytometry protocol that distinguishes viable dividing plasmablasts from nondividing plasma cells and, based on CD19 surface abundance, identifies two mature plasma cell populations in the spleen and the bone marrow of mice.

Keywords: B220; Blimp1; CD138; CD19; Flow cytometry; Long-lived plasma cell; Plasmablast; TACI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD19 / analysis
  • Bone Marrow Cells / immunology
  • Cell Differentiation
  • Flow Cytometry / methods*
  • Green Fluorescent Proteins
  • Immunophenotyping / instrumentation
  • Immunophenotyping / methods*
  • Leukocyte Common Antigens / analysis
  • Mice
  • Plasma Cells / classification*
  • Plasma Cells / immunology*
  • Positive Regulatory Domain I-Binding Factor 1
  • Spleen / immunology
  • Staining and Labeling / methods*
  • Syndecan-1 / analysis
  • Transcription Factors / analysis

Substances

  • Antigens, CD19
  • CD19 antigen, mouse
  • Prdm1 protein, mouse
  • Sdc1 protein, mouse
  • Syndecan-1
  • Transcription Factors
  • Green Fluorescent Proteins
  • Positive Regulatory Domain I-Binding Factor 1
  • Leukocyte Common Antigens