Objective: To study the therapy of cordyceps-astragalus-salvia miltiorrhiza in treating acute lung injury and pulmonary interstitial fibrosis induced by paraquat poisoning. Methods: All 120 adult Wister male rats were randomly assigned to three groups, the paraquat poisoning group (rats were intragastric administration paraquat 50 mg/kg body weight once at the beginning) , the cordyceps-astragalus-salvia miltiorrhiza therapy group (rats were given cordyceps-astragalus-salvia miltiorrhiza 90 mg/kg body weight intragastric administration half an hour after paraquat was given, then the same dose was given once a day) ; control group (rats were intragastric administration with physiological saline) . At 7th, 14th, 21st, and 28th day rats were sacrificed postanesthetic respectively after paraquat exposure, sample of lung tissue, bronchoalveolar lavage (BAL) , and venous blood were collected. GSH, SOD, TNF-α, TGF-β1, and HYP in plasma, bronchoalveolar lavage (BAL) , and the lung homogenates were determined. Optical microscope was performed to examine pathological changes in lung. Results: Each experimental time point paraquat group and the treatment group rats serum SOD content significantly lower than the control group (P<0.05) . Each experimental time point the treatment group rats serum SOD levels increased significantly than that of paraquat group (P<0.05) . Each experimental time point paraquat group rats serum GSH content significantly lower than that of the control group (P<0.05) . Treatment group rats 7 days time GSH content significantly lower than that of the control group (P<0.05) . Treatment group 21 days, 28 days GSH content was increased significantly than that of the paraquat group (P<0.05) . Each experimental time point paraquat group rats alveolar lavage SOD content was significantly lower than that of the control group (P<0.05) . Treatment group 7 days, 14 days time SOD content was significantly lower than that of the control group (P<0.05) , Treatment group 21 days, 28 days SOD content was increased significantly than that of the paraquat group (P<0.05) . Each experimental time point paraquat group and the treatment group rats alveolar lavage GSH content significantly were lower than that of the control group (P<0.05) . Treatment group days 14 and 21 days, 28 days GSH content was increased significantly than that of the paraquat group (P<0.05) . Each experimental time point paraquat group rats alveolar lavage TNF α levels was higher than that of the control group (P<0.05) . Treatment group 7 days, 14 days the rat alveolar lavage TNF α levels was higher than that of the control group (P<0.05) . Treatment group 21 days, 28 days TNF α content significantly was decreased than that of paraquat group (P<0.05) . Paraquat group days 14 and 21 days, 28 days HYP content was significantly higher than that of control group (P<0.05) . Treatment group 21 days HYP content was significantly higher than that of control group (P<0.05) . Treatment group 28 days time HYP content in lung tissue of rats was significantly decreased than that of the paraquat group (P<0.05) . Each experimental time point paraquat group rat lung tissue (tissue homogenate) TGF-β1 content was higher than that of the control group, the difference was statistically significant (P<0.05) . Under optical microscope, the tissue damage of lung was aggravated, and reduced after cordyceps-astragalus-salvia miltiorrhiza was administrated. Conclusion: Cordyceps-astragalus-salvia miltiorrhiza can reduce inflammation factor releasing, and relieve lung injury. It has therapeutic effect on lung injury induced by paraquat poisoning.
目的： 研究虫草芪参胶囊在百草枯诱导的急性肺损伤中的治疗作用。 方法： 将成年雄性Wister大鼠120只，随机分为3组，每组40只。百草枯染毒组，一次性灌胃20%百草枯原液50 mg/kg大鼠体重；百草枯+虫草芪参胶囊治疗组，一次性灌胃20%百草枯原液50 mg/kg大鼠体重，每天给予虫草芪参胶囊混悬液2 ml/kg（相当于虫草芪参胶囊粉剂90 mg/kg）大鼠体重灌胃；空白对照组给予等量生理盐水灌胃。实验结束分别于第7、14、21、28天麻醉后处死大鼠，取静脉血、肺泡灌洗液，检测血清及肺泡灌洗液还原性谷胱甘肽（GSH）、超氧化物歧化酶（SOD）水平，酶联免疫吸附法（ELISA）检测肺泡灌洗液肿瘤坏死因子α（TNF-α）水平。留取大鼠肺组织，检测转化生长因子β1（TGF-β1）和羟脯氨酸（HYP）。留取部分肺组织做病理切片，并做苏木精-伊红（HE）和Masson染色，光学显微镜下观察。 结果： 各实验时间点染毒组和治疗组大鼠血清SOD含量较对照组明显降低，治疗组大鼠血清SOD含量较染毒组明显升高，差异均有统计学意义（P<0.05）。各实验时间点染毒组大鼠血清GSH含量较对照组明显降低，治疗组大鼠7 d时GSH含量较对照组明显降低，治疗组21、28 d时GSH含量较染毒组明显升高，差异均有统计学意义（P<0.05）。各实验时间点染毒组大鼠肺泡灌洗液SOD含量较对照组明显降低，治疗组7、14 d时SOD含量较对照组明显降低，治疗组21、28 d时SOD含量较染毒组明显升高，差异均有统计学意义（P<0.05）。各实验时间点染毒组和治疗组大鼠肺泡灌洗液GSH含量较对照组明显降低，治疗组14、21、28 d时GSH含量较染毒组明显升高，差异均有统计学意义（P<0.05）。各实验时间点染毒组大鼠肺泡灌洗液TNF-α含量较对照组明显升高，治疗组7、14 d时大鼠肺泡灌洗液TNF-α含量较对照组明显升高，治疗组21、28 d时TNF-α含量较染毒组明显降低，差异均有统计学意义（P<0.05）。染毒组14、21、28 d时大鼠肺组织HYP含量较对照组明显升高；治疗组21 d时大鼠肺组织HYP含量较对照组明显升高，治疗组28 d时大鼠肺组织HYP含量较染毒组明显降低，差异均有统计学意义（P<0.05）。各实验时间点染毒组大鼠肺组织（组织匀浆）TGF-β1含量较对照组明显升高，差异有统计学意义（P<0.05）。光学显微镜观察，染毒组肺组织损伤随着实验天数增加而加重，炎症细胞浸润程度逐渐增加，肺泡间隔纤维化在观察后期逐渐形成；治疗组较相同天数染毒组大鼠肺组织炎症程度、纤维化程度轻。 结论： 虫草芪参胶囊有明显的抗氧化作用，能减少细胞炎性因子的释放，减轻肺间质的损伤，治疗百草枯中毒引起的急性肺损伤和肺纤维化有一定疗效。.
Keywords: Cordyceps-astragalus-salvia miltiorrhiza; Lung injury, acute; Paraquat; Pulmonary fibrosis.