Biexponential T_1ρ relaxation mapping of human knee cartilage in vivo at 3 T

The purpose of this study was to demonstrate the feasibility of biexponential T_1ρ relaxation mapping of human knee cartilage in vivo. A three-dimensional, customized, turbo-flash sequence was used to acquire T_1ρ -weighted images from healthy volunteers employing a standard 3-T MRI clinical scanner. A series of T_1ρ -weighted images was fitted using monoexponential and biexponential models with two- and four-parametric non-linear approaches, respectively. Non-parametric Kruskal-Wallis and Mann-Whitney U-statistical tests were used to evaluate the regional relaxation and gender differences, respectively, with a level of significance of P = 0.05. Biexponential relaxations were detected in the cartilage of all volunteers. The short and long relaxation components of T_1ρ were estimated to be 6.9 and 51.0 ms, respectively. Similarly, the fractions of short and long T_1ρ were 37.6% and 62.4%, respectively. The monoexponential relaxation of T_1ρ was 32.6 ms. The experiments showed good repeatability with a coefficient of variation (CV) of less than 20%. A biexponential relaxation model showed a better fit than a monoexponential model to the T_1ρ relaxation decay in knee cartilage. Biexponential T_1ρ components could potentially be used to increase the specificity to detect early osteoarthritis by the measurement of different water compartments and their fractions.