Protocol for improved resolution of plasma cell subpopulations by flow cytometry

Eur J Immunol. 2017 Aug;47(8):1386-1388. doi: 10.1002/eji.201746944.

Abstract

Plasma cells are rare cells that have been notoriously difficult to detect by flow cytometry. New advances have described B220+ CD138+ plasma cells in the bone marrow that are particularly difficult to distinguish between CD138 intermediate B220+ developing B cells. Herein we describe a novel method for detecting plasma cells in the bone marrow using a combination of CD138 and Sca-1 staining.

Keywords: B cells; B220; Blimp-GFP; CD138; Flow cytometry; Plasma cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antigens, Ly / analysis*
  • Bone Marrow Cells / immunology
  • Flow Cytometry / methods*
  • Immunophenotyping / methods*
  • Leukocyte Common Antigens / analysis
  • Membrane Proteins / analysis*
  • Mice
  • Peyer's Patches / cytology
  • Peyer's Patches / immunology
  • Plasma Cells / classification*
  • Plasma Cells / immunology*
  • Positive Regulatory Domain I-Binding Factor 1
  • Spleen / cytology
  • Spleen / immunology
  • Syndecan-1 / analysis*
  • Transcription Factors / analysis

Substances

  • Antigens, Ly
  • Ly6a protein, mouse
  • Membrane Proteins
  • Prdm1 protein, mouse
  • Sdc1 protein, mouse
  • Syndecan-1
  • Transcription Factors
  • Positive Regulatory Domain I-Binding Factor 1
  • Leukocyte Common Antigens