Generation of Macaca fascicularis iPS cell line ATCi-MF1 from adult skin fibroblasts using non-integrative Sendai viruses

Giulia Coppiello; Gloria Abizanda; Natalia Aguado; Elena Iglesias; Estibaliz Arellano-Viera; Juan R Rodriguez-Madoz; Xonia Carvajal-Vergara; Felipe Prosper; Xabier L Aranguren

doi:10.1016/j.scr.2017.03.008

Generation of Macaca fascicularis iPS cell line ATCi-MF1 from adult skin fibroblasts using non-integrative Sendai viruses

Stem Cell Res. 2017 May:21:1-4. doi: 10.1016/j.scr.2017.03.008. Epub 2017 Mar 16.

Authors

Giulia Coppiello¹, Gloria Abizanda¹, Natalia Aguado¹, Elena Iglesias¹, Estibaliz Arellano-Viera¹, Juan R Rodriguez-Madoz¹, Xonia Carvajal-Vergara¹, Felipe Prosper², Xabier L Aranguren³

Affiliations

¹ Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
² Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain; Cell Therapy Area, Clínica Universidad de Navarra, University of Navarra, Health Research Institute of Navarra, Pamplona, Spain. Electronic address: [email protected].
³ Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain. Electronic address: [email protected].

PMID: 28677526
DOI: 10.1016/j.scr.2017.03.008

Abstract

We generated ATCi-MF1 induced pluripotent stem (iPS) cell line from Macaca fascicularis adult skin fibroblasts using non-integrative Sendai viruses carrying OCT3/4, KLF4, SOX2 and c-MYC. Once established, ATCi-MF1 cells present a normal karyotype, are Sendai virus-free and express pluripotency associated markers. Microsatellite markers analysis confirmed the origin of the iPS cells from the parental fibroblasts. Pluripotency was tested with the in vivo teratoma formation assay. ATCi-MF1 cell line may be a useful primate iPS cell model to test different experimental conditions where the use of human cells can imply ethical issues, as microinjection of pluripotent stem cells in pre-implantational embryos.

MeSH terms

Animals
Cell Line
Fibroblasts / cytology
Fibroblasts / metabolism*
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Kruppel-Like Factor 4
Macaca fascicularis
Sendai virus*
Skin / cytology
Skin / metabolism*
Transcription Factors* / biosynthesis
Transcription Factors* / genetics
Transduction, Genetic*

Substances

KLF4 protein, human
Kruppel-Like Factor 4
Transcription Factors