Isolation and characterization of Sprague-Dawley and Wistar Kyoto GFP rat embryonic stem cells

Giulia Coppiello; Gloria Abizanda; Natalia Aguado; Elena Iglesias; Olalla Iglesias-Garcia; Antonio Lo Nigro; Felipe Prosper; Xabier L Aranguren

doi:10.1016/j.scr.2017.03.020

Isolation and characterization of Sprague-Dawley and Wistar Kyoto GFP rat embryonic stem cells

Stem Cell Res. 2017 May:21:40-43. doi: 10.1016/j.scr.2017.03.020. Epub 2017 Mar 29.

Authors

Giulia Coppiello¹, Gloria Abizanda¹, Natalia Aguado¹, Elena Iglesias¹, Olalla Iglesias-Garcia², Antonio Lo Nigro³, Felipe Prosper⁴, Xabier L Aranguren⁵

Affiliations

¹ Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
² Center of Regenerative Medicine in Barcelona (CMRB), Spain.
³ Ri.Med Foundation, Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Palermo, Italy.
⁴ Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain; Cell Therapy Area, Clínica Universidad de Navarra, University of Navarra, Health Research Institute of Navarra, Pamplona, Spain. Electronic address: [email protected].
⁵ Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain. Electronic address: [email protected].

PMID: 28677536
DOI: 10.1016/j.scr.2017.03.020

Abstract

We generated two rat embryonic stem cell (ESC) lines: ATCe-SD7.8 from Sprague-Dawley strain and ATCe-WK1 from Wistar Kyoto strain. Cells were marked with enhanced green fluorescent protein (eGFP) by transduction with a lentiviral vector. Cells present a normal karyotype and express pluripotency-associated markers. Pluripotency was tested in vivo with the teratoma formation assay. Cells maintain eGFP expression upon differentiation to the three-germ layers. These cells can be a useful tool for cell therapy studies and chimera generation as they can be easily tracked by eGFP expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Separation*
Embryonic Stem Cells / cytology
Embryonic Stem Cells / metabolism*
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism*
Rats
Rats, Inbred WKY
Rats, Sprague-Dawley
Rats, Transgenic

Substances

enhanced green fluorescent protein
Green Fluorescent Proteins