Generation of non-transgenic iPS cells from human cord blood CD34+ cells under animal component-free conditions

Alexandra Haase; Gudrun Göhring; Ulrich Martin

doi:10.1016/j.scr.2017.03.022

Generation of non-transgenic iPS cells from human cord blood CD34⁺ cells under animal component-free conditions

Stem Cell Res. 2017 May:21:71-73. doi: 10.1016/j.scr.2017.03.022. Epub 2017 Mar 31.

Authors

Alexandra Haase¹, Gudrun Göhring², Ulrich Martin³

Affiliations

¹ Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany.
² Department of Human Genetics, Hannover Medical School, 30625 Hannover, Germany.
³ Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany. Electronic address: [email protected].

PMID: 28677540
DOI: 10.1016/j.scr.2017.03.022

Abstract

Recently, many hurdles and limitations for production of clinically applicable iPSC derivatives have been overcome. Transgene-free iPSCs can be efficiently derived from easily accessible cell sources such as blood. Here we describe the generation of transgene-free hiPS cells from cord blood derived CD34⁺ cells, reprogrammed using CytoTune™ Sendai reprogramming vectors. CD34⁺ cell isolation, cultivation, reprogramming and establishment of resulting hiPSC lines were performed under the exclusive usage of animal-derived component-free (ADCF) materials and components.

MeSH terms

Antigens, CD34*
Cell Separation*
Cellular Reprogramming Techniques*
Fetal Blood / cytology*
Fetal Blood / metabolism
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism

Substances

Antigens, CD34