Synthesis of 1,3-dioleoyl-2-arachidonoylglycerol-rich structured lipids by lipase-catalyzed acidolysis of microbial oil from Mortierella alpina

Bioresour Technol. 2017 Nov:243:448-456. doi: 10.1016/j.biortech.2017.06.090. Epub 2017 Jun 19.

Abstract

Microbial oils (MOs) have gained widespread attention due to their functional lipids and health promoting properties. In this study, 1,3-dioleoyl-2-arachidonoylglycerol-rich structured lipids (SLs) were produced from MO and oleic acid (OA) via solvent-free acidolysis catalyzed by Lipozyme RM IM. Under the optimal conditions, the content of unsaturated fatty acids (UFAs) increased from 60.63 to 84.00%, while the saturated fatty acids (SFAs) content decreased from 39.37 to 16.00% at sn-1,3 positions in SLs. Compared with MO, arachidonic acid (ARA) content at the sn-2 position of SLs accounted for 49.71%, whereas OA was predominantly located at sn-1,3 positions (47.05%). Meanwhile, the most abundant triacylglycerol (TAG) species in SLs were (18:1-20:4-18:1), (20:4-20:4-18:1), (18:1-18:2-18:1), (18:1-18:2-18:0) and (24:0-20:4-18:1) with a relative content of 18.79%, 11.94%, 6.07%, 5.75% and 4.84%, respectively. Such novel SLs with improved functional properties enriched with UFAs are highly desirable and have the potential to be used in infant formula.

Keywords: Arachidonic acid; Biocatalysis; Microbial oil; Polyunsaturated fatty acids; Structured lipids.

MeSH terms

  • Arachidonic Acids*
  • Endocannabinoids*
  • Glycerides*
  • Humans
  • Lipase
  • Lipids*
  • Mortierella*
  • Oils
  • Triglycerides

Substances

  • Arachidonic Acids
  • Endocannabinoids
  • Glycerides
  • Lipids
  • Oils
  • Triglycerides
  • glyceryl 2-arachidonate
  • Lipase