Biolayer interferometry predicts ELISA performance of monoclonal antibody pairs for Plasmodium falciparum histidine-rich protein 2

Anal Biochem. 2017 Oct 1:534:10-13. doi: 10.1016/j.ab.2017.07.010. Epub 2017 Jul 8.

Abstract

Predicting antibody pair performance in a sandwich format streamlines development of antibody-based diagnostics and laboratory research tools, such as enzyme-linked immunosorbent assays (ELISAs) and lateral flow immunoassays (LFAs). We have evaluated panels of monoclonal antibodies against the malarial parasite biomarker Plasmodium falciparum histidine rich protein 2 (HRP2), including 9 new monoclonal antibodies, using biolayer interferometry (BLI) and screened antibody pairs in a checkerboard ELISA. This study showed BLI predicts antibody pair ELISA performance for HRP2. Pairs that included capture antibodies with low off-rate constants and detection antibodies with high on-rate constants performed best in an ELISA format.

Keywords: Binding kinetics; Biolayer interferometry; ELISA; Histidine-rich protein 2; Malaria.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology*
  • Antigen-Antibody Reactions
  • Antigens, Protozoan / analysis*
  • Antigens, Protozoan / immunology
  • Enzyme-Linked Immunosorbent Assay*
  • Plasmodium falciparum / chemistry*
  • Plasmodium falciparum / immunology
  • Protozoan Proteins / analysis*
  • Protozoan Proteins / immunology

Substances

  • Antibodies, Monoclonal
  • Antigens, Protozoan
  • HRP-2 antigen, Plasmodium falciparum
  • Protozoan Proteins