Abstract
Methanol extracted skins from 84 species of amphibia were screened, measuring by RIAs: gastrin-CCK, VIP, calcitonin, GIP, PP and motilin. G-CCK-like immunoreactivity was found in 97.6%; VIP-like immunoreactivity in 41%; CT-like immunoreactivity in 34%; GIP-like immunoreactivity in 10%; PP-like immunoreactivity in 40% and MT-like immunoreactivity in 60% of the samples. The use of a sequence-specific radioimmunoassay and of gel-chromatography confirmed the caerulein-CCK-8-like nature of the immunoreactive material. Detected amounts of the other peptides (VIP, CT, GIP, PP, MT) were too low for bioassay or chromatographic studies, thus leaving the question open if they are due to some kind of unspecific interferences or, most likely, to species-specificity differences of the used antisera.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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APUD Cells / physiology*
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Animals
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Anura / physiology*
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Calcitonin / analysis
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Calcitonin / immunology
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Cholecystokinin / analysis
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Cholecystokinin / immunology
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Gastric Inhibitory Polypeptide / analysis
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Gastric Inhibitory Polypeptide / immunology
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Gastrins / analysis
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Gastrins / immunology
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Motilin / analysis
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Motilin / immunology
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Pancreatic Polypeptide / analysis
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Pancreatic Polypeptide / immunology
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Peptides / analysis
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Peptides / immunology
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Peptides / physiology*
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Radioimmunoassay
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Skin / analysis
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Skin / immunology
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Skin Physiological Phenomena
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Species Specificity
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Vasoactive Intestinal Peptide / analysis
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Vasoactive Intestinal Peptide / immunology
Substances
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Gastrins
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Peptides
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Vasoactive Intestinal Peptide
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Motilin
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Gastric Inhibitory Polypeptide
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Pancreatic Polypeptide
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Calcitonin
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Cholecystokinin