Comparison of Oropharyngeal and Nasopharyngeal Swab Specimens for the Detection of Mycoplasma pneumoniae in Children with Lower Respiratory Tract Infection

Fujio Kakuya; Takahiro Kinebuchi; Hitoshi Okubo; Kumihiro Matsuo

doi:10.1016/j.jpeds.2017.06.038

Comparison of Oropharyngeal and Nasopharyngeal Swab Specimens for the Detection of Mycoplasma pneumoniae in Children with Lower Respiratory Tract Infection

J Pediatr. 2017 Oct:189:218-221. doi: 10.1016/j.jpeds.2017.06.038. Epub 2017 Jul 13.

Authors

Fujio Kakuya¹, Takahiro Kinebuchi², Hitoshi Okubo³, Kumihiro Matsuo³

Affiliations

¹ Department of Pediatrics, Furano Kyokai Hospital, Furano, Japan. Electronic address: [email protected].
² Clinical Laboratory, Furano Kyokai Hospital, Furano, Japan.
³ Department of Pediatrics, Furano Kyokai Hospital, Furano, Japan.

PMID: 28712517
DOI: 10.1016/j.jpeds.2017.06.038

Abstract

The oropharyngeal swab specimen was superior to the nasopharyngeal swab specimen for the detection of Mycoplasma pneumoniae in children with lower respiratory tract infection. The oropharyngeal loop-mediated isothermal amplification had 100% sensitivity and specificity compared with polymerase chain reaction testing, whereas the oropharyngeal rapid antigen detection test using immunochromatographic assay had relatively low sensitivity (66%) and reasonable specificity (90.7%).

Keywords: diagnostic value; loop-mediated isothermal amplification; rapid antigen detection test; real-time polymerase chain reaction.

Publication types

Comparative Study

MeSH terms

Adolescent
Child
Child, Preschool
Chromatography, Affinity / methods*
Female
Humans
Infant
Male
Mycoplasma pneumoniae / genetics*
Nucleic Acid Amplification Techniques / methods*
Pharynx / microbiology*
Pneumonia, Mycoplasma / diagnosis*
Prospective Studies
Respiratory Tract Infections / diagnosis*
Sensitivity and Specificity