Cancer recurrence monitoring using hyperpolarized [1-13C]pyruvate metabolic imaging in murine breast cancer model

Peter J Shin; Zihan Zhu; Roman Camarda; Robert A Bok; Alicia Y Zhou; John Kurhanewicz; Andrei Goga; Daniel B Vigneron

doi:10.1016/j.mri.2017.07.014

Cancer recurrence monitoring using hyperpolarized [1-¹³C]pyruvate metabolic imaging in murine breast cancer model

Magn Reson Imaging. 2017 Nov:43:105-109. doi: 10.1016/j.mri.2017.07.014. Epub 2017 Jul 15.

Authors

Peter J Shin¹, Zihan Zhu², Roman Camarda³, Robert A Bok⁴, Alicia Y Zhou⁵, John Kurhanewicz², Andrei Goga⁶, Daniel B Vigneron²

Affiliations

¹ Department of Radiology and Biomedical Imaging, University of California at San Francisco, San Francisco, CA, USA. Electronic address: [email protected].
² Department of Radiology and Biomedical Imaging, University of California at San Francisco, San Francisco, CA, USA; The UC Berkeley - UCSF Graduate Program in Bioengineering, CA, USA.
³ Biomedical Sciences Graduate Program, University of California at San Francisco, San Francisco, CA, USA.
⁴ Department of Radiology and Biomedical Imaging, University of California at San Francisco, San Francisco, CA, USA.
⁵ Department of Cell and Tissue Biology, University of California at San Francisco, San Francisco, CA, USA.
⁶ Biomedical Sciences Graduate Program, University of California at San Francisco, San Francisco, CA, USA; Department of Cell and Tissue Biology, University of California at San Francisco, San Francisco, CA, USA.

Abstract

The purpose of this work was to study the anatomic and metabolic changes that occur with tumor progression, regression and recurrence in a switchable MYC-driven murine breast cancer model. Serial ¹H MRI and hyperpolarized [1-¹³C]pyruvate metabolic imaging were used to investigate the changes in tumor volume and glycolytic metabolism over time during the multistage tumorigenesis. We show that acute de-induction of MYC expression in established tumors results in rapid tumor regression and significantly reduced glycolytic metabolism as measured by pyruvate-to-lactate conversion. Moreover, cancer recurrences occurring at the tumor sites independently of MYC expression were observed to accompany markedly increased lactate production.

Keywords: Breast cancer; Cancer recurrence; Hyperpolarized (13)C MRI; Metabolic imaging.

MeSH terms

Animals
Carbon Isotopes
Female
Glycolysis
Humans
Lactic Acid / metabolism*
Magnetic Resonance Imaging*
Magnetic Resonance Spectroscopy*
Mammary Neoplasms, Animal / diagnostic imaging*
Mammary Neoplasms, Animal / metabolism
Mice
Mice, Transgenic
Neoplasm Recurrence, Local
Neoplasm Transplantation
Proto-Oncogene Proteins c-myc / genetics
Pyruvic Acid / metabolism*
Tumor Burden

Substances

Carbon Isotopes
MYC protein, human
Proto-Oncogene Proteins c-myc
Lactic Acid
Pyruvic Acid

Abstract

MeSH terms

Substances

Grants and funding