Prospective evaluation of a monoclonal antibody in diagnosis of Pneumocystis carinii pneumonia

Lancet. 1986 Jul 5;2(8497):1-3. doi: 10.1016/s0140-6736(86)92555-9.

Abstract

To evaluate the ability of a mouse monoclonal antibody, 2G2, directed against human Pneumocystis carinii, to detect the organism in clinical specimens, a prospective study of the antibody in an indirect immunofluorescent assay was undertaken. P carinii was rapidly detected in thirteen of fourteen bronchoalveolar lavage specimens positive by toluidine-blue-O stain, none of eleven lavage specimens negative by toluidine-blue-O, neither of two impression smears of histologically negative open-lung biopsy specimens, and both of two impression smears of histologically positive necropsy specimens. Immunofluorescence with monoclonal antibody 2G2 is a rapid, simple, and specific technique for detection of P carinii in clinical specimens.

MeSH terms

  • Acquired Immunodeficiency Syndrome / complications
  • Animals
  • Antibodies, Monoclonal*
  • Bronchi / microbiology
  • Bronchoscopy
  • Fluorescent Antibody Technique
  • Humans
  • Hybridomas
  • Mice
  • Mice, Inbred BALB C
  • Pneumocystis / immunology
  • Pneumonia, Pneumocystis / diagnosis*
  • Pneumonia, Pneumocystis / immunology
  • Prospective Studies
  • Tolonium Chloride

Substances

  • Antibodies, Monoclonal
  • Tolonium Chloride