MALDI-TOF MS identification and antimicrobial susceptibility testing directly from positive enrichment broth

Marie Tré-Hardy; Barbara Lambert; Noémie Despas; Florian Bressant; Clémentine Laurenzano; Hector Rodriguez-Villalobos; Alexia Verroken

doi:10.1016/j.mimet.2017.07.012

MALDI-TOF MS identification and antimicrobial susceptibility testing directly from positive enrichment broth

J Microbiol Methods. 2017 Oct:141:32-34. doi: 10.1016/j.mimet.2017.07.012. Epub 2017 Jul 25.

Authors

Marie Tré-Hardy¹, Barbara Lambert², Noémie Despas², Florian Bressant², Clémentine Laurenzano², Hector Rodriguez-Villalobos², Alexia Verroken²

Affiliations

¹ Department of Microbiology, Cliniques Universitaires Saint-Luc, Université Catholique de Louvain, Brussels, Belgium. Electronic address: [email protected].
² Department of Microbiology, Cliniques Universitaires Saint-Luc, Université Catholique de Louvain, Brussels, Belgium.

PMID: 28754447
DOI: 10.1016/j.mimet.2017.07.012

Abstract

A rapid preparation procedure was validated for MALDI-TOF MS identification followed by an antimicrobial susceptibility testing directly from a non-selective enrichment broth of sterile fluid samples associated with negative classical cultures. This method can be easily integrated in the laboratory routine allowing precious time gain for microbe identification and subsequent adequate treatment initiation.

Keywords: Biopsies; CSF; MALDI-TOF MS identification; NSEB; Sterile fluid samples.

Publication types

Evaluation Study

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacteria / drug effects*
Bacteria / isolation & purification
Bacteriological Techniques
Humans
Infertility
Liquid Biopsy
Microbial Sensitivity Tests / instrumentation*
Microbial Sensitivity Tests / methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Anti-Bacterial Agents