Effect of dietary supplementation with olive and sunflower oils on lipid profile and liver histology in rats fed high cholesterol diet

Sandra Mara Pimentel Duavy; Gerson Javier Torres Salazar; Gerlânia de Oliveira Leite; Assis Ecker; Nilda Vargas Barbosa

doi:10.1016/j.apjtm.2017.06.001

Effect of dietary supplementation with olive and sunflower oils on lipid profile and liver histology in rats fed high cholesterol diet

Asian Pac J Trop Med. 2017 Jun;10(6):539-543. doi: 10.1016/j.apjtm.2017.06.001. Epub 2017 Jun 15.

Authors

Sandra Mara Pimentel Duavy¹, Gerson Javier Torres Salazar², Gerlânia de Oliveira Leite³, Assis Ecker⁴, Nilda Vargas Barbosa²

Affiliations

¹ Departamento de Química, Universidade Regional do Cariri (URCA), Campus Pimenta, 63105-000 Crato, CE, Brazil.
² Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Campus Universitário - Camobi, 97105-900 Santa Maria, RS, Brazil.
³ Departamento de Farmacologia, Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Campus Universitário - Camobi, 97105-900, Brazil.
⁴ Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Campus Universitário - Camobi, 97105-900 Santa Maria, RS, Brazil. Electronic address: [email protected].

PMID: 28756916
DOI: 10.1016/j.apjtm.2017.06.001

Abstract

Objective: To compare the effects of high-monounsaturated (MUFA) and polyunsaturated fatty acids (PUFA) against the metabolic disorders elicited by a high-cholesterol diet (HC) in rats.

Methods: Using in vivo dietary manipulation, rats were fed with different diets containing 4% soybean oil (cholesterol free diet) and 1% HC containing 12% olive oil (HC + OO) enriched with MUFA and 12% sunflower oil (HC + SO) enriched with PUFA for 60 d. Serum lipid levels and hepatic steatosis were evaluated after the treatment period.

Results: Comparatively, rats treated with HC + OO diet experienced a decrease in the serum LDL-C, VLDL-C and CT levels compared to those fed with HC + SO diet (P < 0.05). Otherwise, HC + OO provoked significant microvesicular steatosis situated in the hepatic acinar zone 1.

Conclusions: HC + OO diet has high absorption velocity in the acinar zone 1 of liver compared to the HC + SO diet. Based on this, the reduction of the LDL-C, VLDL-C and CT serum levels in the animals treated with HC + OO diet may have been caused by the delay in the FA release to the blood.

Keywords: Cholesterol diets; Liver steatosis; Olive oil; Serum lipids; Sunflower oil.