Effectivity of oral recombinant DNA vaccine against Streptococcus agalactiae in Nile tilapia

Dev Comp Immunol. 2017 Dec:77:77-87. doi: 10.1016/j.dci.2017.07.024. Epub 2017 Jul 28.

Abstract

The effects of oral vaccination by two immune routes (gavage and mixed fodder administration) using a recombinant DNA vaccine SL7207-pVAX1-sip against Streptococcus agalactiae were assessed. SL7207-pVAX1-sip significantly enhanced various innate immune responses of tilapia, such as total serum protein, superoxide dismutase activity, lysozyme activity, complement C3 concentration in serum, serum antibacterial activity, and interleukin 1β and tumor necrosis factor-α. Vaccinated fish had higher relative percent survival values (57% and 63% for gavage group and mix fodder administration, respectively) at 10 weeks after S. agalactiae infection. When administered mixed fodder, the DNA vaccine SL7207-pVAX1-sip against S. agalactiae may produce more effective protection. These findings can promote the application and development of DNA vaccines in aquaculture.

Keywords: DNA vaccine; Immune response; Nile tilapia; S. agalactiae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Bacterial Vaccines / immunology*
  • Complement C3 / metabolism
  • Drug Administration Routes
  • Fish Diseases / immunology*
  • Fish Proteins / metabolism
  • Immunity, Innate
  • Interleukin-1beta / metabolism
  • Muramidase / metabolism
  • Streptococcal Infections / immunology*
  • Streptococcus agalactiae / immunology*
  • Superoxide Dismutase / metabolism
  • Tilapia / immunology*
  • Tilapia / microbiology
  • Tumor Necrosis Factor-alpha / metabolism
  • Up-Regulation
  • Vaccination
  • Vaccines, DNA

Substances

  • Bacterial Vaccines
  • Complement C3
  • Fish Proteins
  • Interleukin-1beta
  • Tumor Necrosis Factor-alpha
  • Vaccines, DNA
  • Superoxide Dismutase
  • Muramidase