Safflower (Carthamus tinctorius L.) oil during pregnancy and lactation influences brain excitability and cortex oxidative status in the rat offspring

Nutr Neurosci. 2018 Dec;21(10):753-760. doi: 10.1080/1028415X.2017.1360549. Epub 2017 Aug 7.

Abstract

Objectives: To evaluate how safflower oil (SFO) influences brain electrophysiology and cortical oxidative status in the offspring, mothers received a diet with SFO during brain development period.

Methods: Beginning on the 14th day of gestation and throughout lactation, rats received safflower (safflower group - SG) or soybean oil (control group - CG) in their diet. At 65 days old, cortical spreading depression (CSD) and cortex oxidative status were analyzed in the offspring.

Results: SG presented reduction of the CSD velocity as compared to the CG (SG: 3.24 ± 0.09; CG: 3.37 ± 0.07 mm/min). SFO reduced levels of lipid peroxidation by 39.4%. SG showed the following increases: glutathione-S-transferase, 40.8% and reduced glutathione, 34.3%. However, SFO decreased superoxide dismutase by 40.4% and catalase by 64.1%. To control for interhemispheric effects, since CSD was recorded only in the right cortex, we evaluated the oxidative status in both sides of the cortex; no differences were observed.

Discussion: Data show that when SFO is consumed by the female rats during pregnancy and lactation, the offspring present long-term effects on brain electrophysiology and cortical oxidative state. The present study highlights the relevance of understanding the SFO intake of pregnant and lactating mammals.

Keywords: Brain development; Brain oxidative state; Cortical spreading depression; Safflower oil.

MeSH terms

  • Animals
  • Brain / drug effects*
  • Brain / metabolism
  • Carthamus tinctorius / chemistry*
  • Catalase / metabolism
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / metabolism
  • Cortical Spreading Depression / drug effects
  • Female
  • Glutathione / metabolism
  • Lactation*
  • Lipid Peroxidation / drug effects
  • Oxidation-Reduction / drug effects
  • Oxidative Stress / drug effects
  • Pregnancy
  • Rats
  • Rats, Wistar
  • Safflower Oil / pharmacology*
  • Superoxide Dismutase / metabolism

Substances

  • Safflower Oil
  • Catalase
  • Superoxide Dismutase
  • Glutathione