Investigating the susceptibility of mice to a bacterial challenge after intravenous exposure to durable nanoparticles

Nanomedicine (Lond). 2017 Sep;12(17):2097-2111. doi: 10.2217/nnm-2017-0176. Epub 2017 Aug 14.

Abstract

Aim: The goal of this study was to determine whether bacterial clearance in a rodent model would be impaired upon exposure to gold, silver or silica nanoparticles (NPs).

Materials & methods: Mice received weekly injections of NPs followed by a challenge of Listeria monocytogenes (LM). On days 3 and 10 after LM injections, the animals were sacrificed and their tissues were collected for elemental analysis, electron microscopy and LM count determination.

Results: The untreated and NP-treated animals cleared LM at the same rate suggesting that bioaccumulation of NPs did not increase the animals' susceptibility to bacterial infection.

Conclusion: The data from this study indicate that the bioaccumulation of NPs does not significantly affect the ability to react to a bacterial challenge.

Keywords: bioaccumulation; biopersistence; durable nanoparticles; host resistance; macrophage function.

MeSH terms

  • Administration, Intravenous
  • Animals
  • Cell Survival
  • Female
  • Gold / chemistry
  • Humans
  • Listeria monocytogenes / drug effects*
  • Listeriosis / drug therapy*
  • Listeriosis / metabolism
  • Listeriosis / microbiology
  • Macrophages / cytology
  • Macrophages / drug effects
  • Mice
  • Mice, Inbred BALB C
  • Nanoparticles / chemistry*
  • Particle Size
  • RAW 264.7 Cells
  • Reactive Oxygen Species / metabolism
  • Silicon Dioxide / chemistry
  • Silver / chemistry
  • Surface Properties
  • Tissue Distribution

Substances

  • Reactive Oxygen Species
  • Silver
  • Gold
  • Silicon Dioxide