Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification

Methods Mol Biol. 2017:1660:105-110. doi: 10.1007/978-1-4939-7253-1_9.

Abstract

Exosomes were originally described 29 years ago as a mechanism for the removal of redundant molecules from reticulocytes, nothing but a process of removing cellular trash. It is now however, abundantly clear that exosomes have a more significant biological role. However, there is currently limited information pertaining to efficient isolation procedures that can be used to isolate exosomes from both cell culture media, and clinical samples such as plasma. Here, we present a reliable and efficient procedure that can be utilized for the isolation of exosomes from various starting materials.

Keywords: Cell culture supernatant; Exosomes; Extracellular vesicles; Human plasma; Isolation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Fractionation / methods*
  • Chromatography, Gel* / methods
  • Culture Media, Conditioned / chemistry
  • Exosomes* / chemistry
  • Extracellular Vesicles / chemistry
  • Humans
  • Plasma / chemistry

Substances

  • Culture Media, Conditioned