Abstract
Inosinate dehydrogenase (IMP-dehydrogenase) from Chinese Hamster Ovary cells was found to be inhibited by two types of inhibitors. One type of inhibitor binds competitively at IMP-binding site and other type at NAD-binding site of the enzyme. Some new inhibitors of both classes have been illustrated here and their inhibition constants and type of inhibition determined. NAD-analog of tiazofurin was found to bind with both the sites and this type of inhibitors were found to be non-competitive with IMP and NAD.
MeSH terms
-
Allosteric Site
-
Animals
-
Binding Sites
-
Binding, Competitive
-
Cell Line
-
Cricetinae
-
Cricetulus
-
Female
-
Fibroblasts / enzymology
-
IMP Dehydrogenase / antagonists & inhibitors*
-
Ketone Oxidoreductases / antagonists & inhibitors*
-
NAD / analogs & derivatives
-
NAD / metabolism
-
Ovary
-
Protein Binding
-
Ribavirin / analogs & derivatives
-
Ribavirin / metabolism
-
Ribavirin / pharmacology
-
Ribonucleotides / chemical synthesis
-
Ribonucleotides / pharmacology
Substances
-
Ribonucleotides
-
NAD
-
Ribavirin
-
IMP Dehydrogenase
-
Ketone Oxidoreductases
-
tiazofurin