Parathyroid hormone-related protein purified from a human lung cancer cell line

Proc Natl Acad Sci U S A. 1987 Jul;84(14):5048-52. doi: 10.1073/pnas.84.14.5048.

Abstract

A protein with biological activities similar to parathyroid hormone (PTH) has been purified from serum-free culture medium obtained from a human lung cancer cell line (BEN). A major protein band of 18 kDa was obtained on NaDodSO4/polyacrylamide gels, with faint bands at 35 kDa and 67 kDa. Biological activity was associated only with the 18-kDa band. Amino acid sequence analysis of the material purified by HPLC revealed that 8 of the 16 residues were identical with those of human PTH. Antibody raised to a corresponding synthetic peptide recognized the PTH-related material but showed less than 1% cross-reactivity with human PTH amino-terminal peptides. BEN cells contained PTH DNA, but not PTH messenger RNA, indicating involvement of another gene. The purified PTH-related protein had a specific biological activity approximately equal to 6 times greater than that of bovine PTH(1-34). The PTH-related protein may have a role in the syndrome of humoral hypercalcemia of malignancy.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carcinoma, Squamous Cell / analysis*
  • Carcinoma, Squamous Cell / complications
  • Cell Line
  • Cross Reactions
  • Cyclic AMP / biosynthesis
  • DNA, Neoplasm / analysis
  • Hormones, Ectopic / immunology
  • Hormones, Ectopic / isolation & purification*
  • Hormones, Ectopic / pharmacology
  • Humans
  • Hypercalcemia / etiology
  • Lung Neoplasms / analysis*
  • Lung Neoplasms / complications
  • Neoplasm Proteins / immunology
  • Neoplasm Proteins / isolation & purification*
  • Neoplasm Proteins / pharmacology
  • Paraneoplastic Endocrine Syndromes / etiology
  • Parathyroid Hormone / immunology
  • Parathyroid Hormone / pharmacology
  • Parathyroid Hormone-Related Protein
  • Peptide Fragments / immunology
  • Peptide Fragments / pharmacology
  • Peptides / chemical synthesis
  • Peptides / immunology
  • Poly A / analysis
  • RNA, Messenger / analysis
  • RNA, Neoplasm / analysis
  • Teriparatide

Substances

  • DNA, Neoplasm
  • Hormones, Ectopic
  • Neoplasm Proteins
  • PTHLH protein, human
  • Parathyroid Hormone
  • Parathyroid Hormone-Related Protein
  • Peptide Fragments
  • Peptides
  • RNA, Messenger
  • RNA, Neoplasm
  • Teriparatide
  • Poly A
  • Cyclic AMP