Photoswitchable Janus glycodendrimer micelles as multivalent inhibitors of LecA and LecB from Pseudomonas aeruginosa

Colloids Surf B Biointerfaces. 2017 Nov 1:159:605-612. doi: 10.1016/j.colsurfb.2017.08.016. Epub 2017 Aug 16.

Abstract

The first example of the self-assembly and lectin binding properties of photoswitchable glycodendrimer micelles is reported. Light-addressable micelles were assembled from a library of 12 amphiphilic Janus glycodendrimers composed of variable carbohydrate head groups and hydrophobic tail groups linked to an azobenzene core. Spontaneous association in water gave cylindrical micelles with uniform size distribution as determined by dynamic light scattering (DLS) and small angle neutron scattering (SANS). Trans-cis photoisomerization of the azobenzene dendrimer core was used to probe the self-assembly behaviour and lectin binding properties of cylindrical micelles, revealing moderate-to-potent inhibition of lectins LecA and LecB from Pseudomonas aeruginosa.

Keywords: Janus glycodendrimer; Lectin inhibitors; Photoswitchable amphiphiles.

MeSH terms

  • Adhesins, Bacterial / metabolism*
  • Azo Compounds / chemistry
  • Azo Compounds / pharmacology
  • Dendrimers / chemistry*
  • Dynamic Light Scattering
  • Hydrophobic and Hydrophilic Interactions
  • Lectins / antagonists & inhibitors
  • Lectins / chemistry*
  • Lectins / metabolism
  • Micelles
  • Pseudomonas aeruginosa / drug effects*
  • Pseudomonas aeruginosa / metabolism*
  • Scattering, Small Angle

Substances

  • Adhesins, Bacterial
  • Azo Compounds
  • Dendrimers
  • LecA protein, bacteria
  • LecB protein, Pseudomonas aeruginosa
  • Lectins
  • Micelles
  • azobenzene