From Vegetable Waste to New Agents for Potential Health Applications: Antioxidant Properties and Effects of Extracts, Fractions and Pinocembrin from Glycyrrhiza glabra L. Aerial Parts on Viability of Five Human Cancer Cell Lines

J Agric Food Chem. 2017 Sep 13;65(36):7944-7954. doi: 10.1021/acs.jafc.7b03045. Epub 2017 Sep 1.

Abstract

Glycyrrhiza glabra cultivation and harvesting produces substantial quantities of aerial parts as waste. With the aim to prospect an innovative valorization of these byproducts, the aerial parts were harvested in May and October and analyzed for their chemical profile, antioxidant properties, and effects on viability of five cancer cell lines. Pinocembrin was the main constituent. A significant protection of lipid peroxidation was observed with the May total extract (IC50 of 4.2 ± 0.4 μg/mL at 30 min of incubation). The effects on viability of HeLa, MCF-7, MDA-MB-231, Caco-2, and PC3 human cancer cells were investigated. All samples shown a remarkable activity with IC50 values below 25 μg/mL. Samples from plants harvested in May exhibited greater activity than those harvested in October. MCF-7 and HeLa were the most sensitive cells with IC50 in the range 2.73-3.01 and 3.28-5.53 μg/mL, respectively. G. glabra aerial parts represent a good source of valuable products.

Keywords: Glycyrrhiza glabra; antioxidant properties; effects on cellular viability; human cancer cells; vegetable waste.

MeSH terms

  • Antioxidants / chemistry
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology*
  • Cell Line, Tumor
  • Cell Survival / drug effects*
  • Flavanones / chemistry
  • Flavanones / isolation & purification
  • Flavanones / pharmacology*
  • Glycyrrhiza / chemistry*
  • Humans
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Vegetables / chemistry*
  • Waste Products / analysis

Substances

  • Antioxidants
  • Flavanones
  • Plant Extracts
  • Waste Products
  • pinocembrin