Expression and purification of classical swine fever virus E2 protein from Sf9 cells using a modified vector

Biotechnol Lett. 2017 Dec;39(12):1821-1825. doi: 10.1007/s10529-017-2426-y. Epub 2017 Sep 1.

Abstract

Objective: To develop a simple method for efficient expression of classical swine fever virus (CSFV) E2 protein.

Results: The pFastBac HT B vector (pFastHTB-M1) was modified by adding a melittin signal peptide sequence. The E2 gene fragment without the transmembrane region was cloned into pFastHTB-M1. The modified vector has clear advantage over the original one, as evidenced by the purified recombinant E2 protein that was detected significantly by SDS-PAGE.

Conclusions: The modified vector has the potential for large-scale production and easy purification of the CSFV E2 protein or other proteins of interests.

Keywords: Baculovirus expression system; Classical swine fever virus; E2 protein; Secretory expression.

MeSH terms

  • Animals
  • Baculoviridae / genetics
  • Genetic Vectors / genetics*
  • Protein Engineering / methods*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics*
  • Recombinant Proteins / isolation & purification*
  • Recombinant Proteins / metabolism
  • Sf9 Cells
  • Swine
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / genetics*
  • Viral Envelope Proteins / isolation & purification*
  • Viral Envelope Proteins / metabolism

Substances

  • Recombinant Proteins
  • Viral Envelope Proteins
  • glycoprotein E2, classical swine fever virus