Candida-reactive T cells for the diagnosis of invasive Candida infection of the lumbar vertebral spine

Mycoses. 2018 Jan;61(1):48-52. doi: 10.1111/myc.12696. Epub 2017 Sep 26.

Abstract

Invasive Candida infection is the fourth most common bloodstream infection. Blood cultures are the current gold standard diagnostic method, however, false negatives remain a clinical challenge. We developed a new technique measuring Candida-reactive T cells as diagnostic read-out for invasive Candida infection. In a pilot study, we followed the treatment course of a patient with an invasive Candida infection of the lumbar vertebral spine. We present the case of a 56-year-old patient with HIV-associated Burkitt lymphoma who developed septic shock during chemotherapy-induced neutropenia. For the first time, we provide flow cytometry-based diagnostics with Candida-reactive T cells for invasive candidiasis with comprehensive MRI imaging. The Candida-reactive T cell assay has potential to complement current diagnostic assays for invasive Candida infection and thus to support targeted treatment.

Keywords: CD154; Candida spondylodiscitis; candidaemia; flow cytometry; invasive candidiasis; osteomyelitis.

Publication types

  • Case Reports

MeSH terms

  • Burkitt Lymphoma / complications
  • Burkitt Lymphoma / virology
  • C-Reactive Protein / analysis
  • CD40 Ligand / analysis
  • CD40 Ligand / immunology
  • Candida / immunology*
  • Candidiasis, Invasive / blood
  • Candidiasis, Invasive / diagnosis*
  • Candidiasis, Invasive / immunology*
  • Discitis / microbiology
  • Flow Cytometry / methods
  • HIV Infections / complications
  • Humans
  • Lumbar Vertebrae / microbiology*
  • Male
  • Middle Aged
  • Neutropenia / complications
  • Neutropenia / microbiology
  • Osteomyelitis / diagnosis
  • Osteomyelitis / microbiology
  • Pilot Projects
  • T-Lymphocytes / immunology*

Substances

  • CD40 Ligand
  • C-Reactive Protein