Triazolium cyclodextrin click cluster-resin conjugate: an enrichment material for phosphatidylinositol (3,4,5)-triphosphate

H T Le; J W Lee; S C Park; J W Jeong; W Jung; C W Lim; K P Kim; T W Kim

doi:10.1039/c7cc06151j

Triazolium cyclodextrin click cluster-resin conjugate: an enrichment material for phosphatidylinositol (3,4,5)-triphosphate

Chem Commun (Camb). 2017 Sep 25;53(75):10459-10462. doi: 10.1039/c7cc06151j. Epub 2017 Sep 11.

Authors

H T Le¹, J W Lee², S C Park², J W Jeong¹, W Jung³, C W Lim⁴, K P Kim², T W Kim¹

Affiliations

¹ Graduate School of East-West Medical Science, Kyung Hee University, Yongin, 17104, Republic of Korea. [email protected].
² Department of Applied Chemistry, College of Applied Science, Kyung Hee University, Yongin, 17104, Republic of Korea. [email protected].
³ Department of Emergency Medicine, School of Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea.
⁴ Department of Chemistry, College of Life Science and Nano-technology, Hannam University, Daejeon, 34430, Republic of Korea.

PMID: 28890969
DOI: 10.1039/c7cc06151j

Abstract

UltraLink was functionalized with a triazolium cyclodextrin click cluster (CCC) which provides a well-oriented, multivalent, positively charged binding site for PtdIns(3,4,5)P₃. MALDI TOF MS and LC ESI MS/MS MRM analysis of spiked PtdIns(3,4,5)P₃ in lipid extract suggest that triazolium CCC-UltraLink conjugate can be used as an enrichment material for PtdIns(3,4,5)P₃.