Whole-genome sequencing identification of a multidrug-resistant Salmonella enterica serovar Typhimurium strain carrying blaNDM-5 from Guangdong, China

Xiaoyan Li; Yan Jiang; Kuihai Wu; Yong Zhou; Ruike Liu; Yan Cao; Aiwu Wu; Yurong Qiu

doi:10.1016/j.meegid.2017.09.005

Whole-genome sequencing identification of a multidrug-resistant Salmonella enterica serovar Typhimurium strain carrying bla_NDM-5 from Guangdong, China

Infect Genet Evol. 2017 Nov:55:195-198. doi: 10.1016/j.meegid.2017.09.005. Epub 2017 Sep 8.

Authors

Xiaoyan Li¹, Yan Jiang², Kuihai Wu³, Yong Zhou⁴, Ruike Liu⁵, Yan Cao⁶, Aiwu Wu⁷, Yurong Qiu⁸

Affiliations

¹ Laboratory Medicine Centre, Nanfang Hospital, Southern Medical University, Guangzhou, China; Department of Laboratory Medicine, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
² Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
³ Department of Laboratory Medicine, First People's Hospital of Foshan, Foshan, China.
⁴ Guangzhou Center for Disease Control and Prevention, Guangzhou, China.
⁵ Department of Endocrinology, Third People's Hospital of Dongguan, Dongguan, China.
⁶ Department of Emergence, Hunan Provincial People's Hospital, Changsha, China.
⁷ KingMed College of Laboratory Medicine, Guangzhou Medical University, Guangzhou, China.
⁸ Laboratory Medicine Centre, Nanfang Hospital, Southern Medical University, Guangzhou, China; Key Laboratory of Biochip Technology in Guangdong Province, Guangzhou, China. Electronic address: [email protected].

PMID: 28893688
DOI: 10.1016/j.meegid.2017.09.005

Abstract

A carbapenem-resistant Salmonella enterica serovar Typhimurium (sequence type 34 [ST34]) strain was isolated from a fecal specimen from a child with acute diarrhea. Whole-genome sequencing revealed that the 84.5-kb IncFII plasmid pST41-NDM carrying the NDM-5 carbapenemase gene possesses a structure identical to that of the IncFII-type plasmid backbone. However, the bla_NDM-5 flanking sequence found in this plasmid is identical to the bla_NDM-5-positive IncX3 plasmids carried by 10 strains of Enterobacteriaceae identified in the same hospital.

Keywords: Insertion sequence; NDM-5 carbapenemase; Plasmid; Salmonella enterica serovar Typhimurium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

China
Computational Biology / methods
Drug Resistance, Multiple, Bacterial*
Genome, Bacterial*
Genomics* / methods
Humans
Mutagenesis, Insertional
Plasmids / genetics
Salmonella Infections / microbiology
Salmonella typhimurium / drug effects*
Salmonella typhimurium / genetics*
Whole Genome Sequencing*
beta-Lactamases / genetics*

Substances

beta-Lactamases