SJL bone marrow-derived macrophages do not have IRF3 mutations and are highly susceptible to Theiler's virus infection

Virology. 2017 Dec:512:21-24. doi: 10.1016/j.virol.2017.08.038.

Abstract

It is well known that SJL mice are susceptible to Theiler's murine encephalomyelitis virus (TMEV)-induced demyelinating disease while C57BL6 (B6) and B10 mice are resistant, and H-2s on a B10 background (B10.S) contributes modestly to susceptibility. A recent study linked two IRF3 non-conservative mutations in SJL compared to B10.S mice to resistance to TMEV infection of SJL peritoneal-derived macrophages, an observation of practical interest in light of the central role of IRF3 transcription factor in the type I interferon (IFN) response. However, we did not find these non-conservative mutations among SJL, B10.S, B6 and B10 mice in the IRF3 amino acid sequence, and show SJL bone marrow-derived macrophages infected with TMEV exhibit increased virus RNA replication and infectious virus yields as well as greater IL-6 production than C57Bl strain (including B10.S) cultures.

Keywords: IRF3; Macrophages; Picornavirus; Theiler's virus; Virus persistence.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cardiovirus Infections / virology*
  • Cells, Cultured
  • Gene Expression Regulation / physiology
  • Genetic Predisposition to Disease*
  • Interferon Regulatory Factor-3 / genetics
  • Interferon Regulatory Factor-3 / metabolism*
  • Interleukin-6 / genetics
  • Interleukin-6 / metabolism
  • Macrophages / metabolism*
  • Mice
  • Mice, Inbred Strains
  • Mutation
  • Theilovirus / physiology*

Substances

  • Interferon Regulatory Factor-3
  • Interleukin-6
  • Irf3 protein, mouse