Insect Bite Hypersensitivity in Horses is Associated with Airway Hyperreactivity

J Vet Intern Med. 2017 Nov;31(6):1877-1883. doi: 10.1111/jvim.14817. Epub 2017 Sep 18.

Abstract

Background: Genetic and epidemiologic evidence suggests that in horses, as in other species, different manifestations of hypersensitivity may occur together.

Hypothesis: Horses affected with insect bite hypersensitivity (IBH) show airway hyperreactivity (AH) to inhaled histamine, even in the absence of overt clinical signs of equine asthma (EA).

Animals: Twenty-two healthy controls (group C), 24 horses suffering from IBH alone (group IBH), and 23 horses suffering from IBH and EA (group IBH/EA).

Methods: The clinical histories were assessed using 2 standardized questionnaires, the Horse Owner Assessed Respiratory Signs Index (HOARSI), and IBH scoring. Horses were classified as EA-affected if their HOARSI was >1 and as IBH-affected if IBH score was >0. Confounding disorders were excluded by clinical examination. The arterial partial pressure of oxygen (PaO2 ) was measured and flowmetric plethysmography used to assess airway reactivity to increasing doses of inhaled histamine.

Results: The median histamine provocation concentration (PC) when ∆flow values increased by 35% (PC35) was significantly higher in group C (5.94 [1.11-26.33] mg/mL) compared to group IBH (2.95 [0.23-10.13] mg/mL) and group IBH/EA (2.03 [0.43-10.94] mg/mL; P < 0.01). The PC50 and PC75 showed very similar differences between groups. Furthermore, PaO2 was significantly lower in group IBH (84 ± 8 mmHg) and group IBH/EA (78 ± 11 mmHg) compared to group C (89 ± 6 mmHg; P < 0.01).

Conclusions and clinical importance: IBH is associated with AH and decreased PaO2 , even in the absence of overt respiratory clinical signs.

Keywords: Equine asthma; Horse; Insect bite hypersensitivity; Multiple equine allergies.

MeSH terms

  • Animals
  • Asthma / veterinary
  • Bronchial Hyperreactivity / chemically induced
  • Bronchial Hyperreactivity / veterinary*
  • Female
  • Histamine / administration & dosage
  • Horse Diseases / diagnosis*
  • Horse Diseases / immunology
  • Horse Diseases / pathology
  • Horses
  • Hypersensitivity / immunology
  • Hypersensitivity / veterinary*
  • Insect Bites and Stings / immunology
  • Insect Bites and Stings / veterinary*
  • Male
  • Oxygen / metabolism
  • Plethysmography / veterinary
  • Switzerland

Substances

  • Histamine
  • Oxygen