Occurrence of antibiotics in mussels and clams from various FAO areas

Luca Maria Chiesa; Maria Nobile; Renato Malandra; Sara Panseri; Francesco Arioli

doi:10.1016/j.foodchem.2017.07.072

Occurrence of antibiotics in mussels and clams from various FAO areas

Food Chem. 2018 Feb 1:240:16-23. doi: 10.1016/j.foodchem.2017.07.072. Epub 2017 Jul 17.

Authors

Luca Maria Chiesa¹, Maria Nobile¹, Renato Malandra², Sara Panseri³, Francesco Arioli¹

Affiliations

¹ Department of Health, Animal Science and Food Safety, University of Milan, Via Celoria 10, 20133 Milan, Italy.
² ATS Milano-Città metropolitana, Director of Veterinary Unit, Via Celoria 10, 20133 Milan, Italy.
³ Department of Health, Animal Science and Food Safety, University of Milan, Via Celoria 10, 20133 Milan, Italy. Electronic address: [email protected].

PMID: 28946257
DOI: 10.1016/j.foodchem.2017.07.072

Abstract

Filter feeders, like mussels and clams, are suitable bioindicators of environmental pollution. These shellfish, when destined for human consumption, undergo a depuration step that aims to nullify their pathogenic microorganism load and decrease chemical contamination. Nevertheless, the lack of contamination by drugs may not be guaranteed. Antimicrobials are a class of drugs of particular concern due to the increasing phenomenon of antibiotic resistance. Their use in breeding and aquaculture is a major cause of this. We developed a multiclass method for the HPLC-MS/MS analysis of 29 antimicrobials, validated according to the Commission Decision 2002/657/UE guidelines, and applied it to 50 mussel and 50 clam samples derived from various Food and Agricultural Organisation marine zones. The results obtained, indicate a negligible presence of antibiotics. Just one clam sample showed the presence of oxytetracycline at a concentration slightly higher than the European Union Maximum residue limit set for fish.

Keywords: Amoxicillin (PubChem CID: 33613); Ampicillin (PubChem CID: 6249); Antibiotics; Benzylpenicillin (PubChem CID: 5904); Cefalexin (PubChem CID: 27447); Cefquinome sulphate (PubChem CID: 9577261); Chloramphenicol (PubChem CID: 5959); Chlortetracycline (PubChem CID: 54737570); Ciprofloxacin (PubChem CID: 2764); Clam; Cloxacillin (PubChem CID: 6098); Dicloxacillin (PubChem CID: 18381); Doxycycline hyclate (PubChem CID: 54686183); Enrofloxacin (PubChem CID: 71188); Erythromycin (PubChem CID: 12560); Florfenicol (PubChem CID: 114811); Florfenicol amine (PubChem CID: 156406); Flumequine (PubChem CID: 3374); HPLC–MS/MS; Lincomycin (PubChem CID: 3000540); Lomefloxacin hydrochloride (PubChem CID: 68624); Marbofloxacin (PubChem CID: 60651); Mussel; Nalidixic acid (PubChem CID: 4421); Oxolinic acid (PubChem CID: 4628); Oxytetracycline (PubChem CID: 54675779); Sulphadiazine (PubChem CID: 441244); Sulphadimethoxine (PubChem CID: 5323); Sulphadimidine (PubChem CID: 5327); Sulphathiazole (PubChem CID: 5340); Tetracycline hydrochloride (PubChem CID: 54704426); Trimethoprim (PubChem CID: 5578); Tylosin (PubChem CID: 5280440).

MeSH terms

Animals
Anti-Bacterial Agents / analysis*
Aquaculture
Bivalvia*
Tandem Mass Spectrometry

Substances

Anti-Bacterial Agents