Cabazitaxel-conjugated nanoparticles for docetaxel-resistant and bone metastatic prostate cancer

Cancer Lett. 2017 Dec 1:410:169-179. doi: 10.1016/j.canlet.2017.09.029. Epub 2017 Sep 28.

Abstract

Effective treatment of metastatic castration resistant prostate cancer (mCRPC) remains an unmet challenge. Cabazitaxel (CBZ) is approved for mCRPC after docetaxel (DTX) failure, but the improvement in survival is only moderate (∼2 months) and patients suffer from significant side effects. Here, we report the development of a polymer based delivery system for CBZ to improve its safety and efficacy against DTX-resistant mCRPC. CBZ was conjugated to a carboxymethylcellulose-based polymer (Cellax-CBZ), which self-assembled into ∼100 nm particles in saline and exhibited sustained drug release in serum at 10%/day. Cellax-CBZ delivered 157-fold higher CBZ to PC3-RES prostate tumor in mice and could be safely administered at a 25-fold higher dose compared to free CBZ, resulting in superior tumor inhibition in multiple mice models of DTX-resistant CRPC. In a metastatic bone model of CRPC, Cellax-CBZ significantly improves overall survival with a 70% long-term survival rate to day 120, while mice treated with free CBZ had a median survival of 40 days. Cellax-CBZ induced mild and reversible neutropenia in mice but no other tissue damage. Cellax-CBZ showed significant potential for improving therapy of mCRPC over clinically approved CBZ.

Keywords: Bone metastasis; Cabazitaxel; Drug-resistance; Nanoparticles; Prostate cancer.

MeSH terms

  • Animals
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacokinetics
  • Antineoplastic Agents / pharmacology*
  • Antineoplastic Agents / toxicity
  • Bone Neoplasms / drug therapy*
  • Bone Neoplasms / secondary
  • Carboxymethylcellulose Sodium / chemistry
  • Cell Line, Tumor
  • Delayed-Action Preparations
  • Docetaxel
  • Drug Carriers
  • Drug Compounding
  • Drug Liberation
  • Drug Resistance, Neoplasm*
  • Humans
  • Male
  • Maximum Tolerated Dose
  • Mice, Inbred NOD
  • Mice, SCID
  • Nanoparticles*
  • Neutropenia / chemically induced
  • Particle Size
  • Prostatic Neoplasms, Castration-Resistant / drug therapy*
  • Prostatic Neoplasms, Castration-Resistant / pathology
  • Solubility
  • Taxoids / chemistry
  • Taxoids / pharmacokinetics
  • Taxoids / pharmacology*
  • Taxoids / toxicity
  • Tissue Distribution
  • Xenograft Model Antitumor Assays

Substances

  • Antineoplastic Agents
  • Delayed-Action Preparations
  • Drug Carriers
  • Taxoids
  • Docetaxel
  • cabazitaxel
  • Carboxymethylcellulose Sodium

Grants and funding